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病毒

人磷酸酶基因Cas9敲除文库

文字:[大][中][小] 手机页面二维码 2016/8/30     浏览次数:    


WX125载体信息


WX125是克隆gRNA的慢病毒载体,上面带有荧光和抗性。

我们提供的一个包装包含1.6ml的病毒2支,滴度在1E+07 TU/mL左右。

这些慢病毒主要用来建立表达gRNA的稳定株,spCas9通过另外的病毒感染进入。目前我们已经有AAV载体的spCas9, 细胞上的实验主要用DJ血清型。在感染spCas9之后两天开始,spCas9-gRNA切割并破坏目的基因,直到随着细胞分裂,AAV载体丢失,这种作用才会停止。

图翎生物正在建立利用Crispr/Cas9系统开发人类基因组敲除文库平台,人的磷酸酶家族基因是我们推出的第一个敲除文库产品,其他文库的建立正在进行中,有需要的用户可以咨询合作。



提示: 请按ctrl+F 输入您要查询的病毒(更多病毒信息请来电或邮件咨询)


来源 编号 磷酸酶 靶点序列 靶点大小 敲除转录本信息
TULYM-TT 1 PTPRA AGAGTCTGAAGAATTGACAG 233bp 3/3 NM_002836.3
NM_080840.2 
NM_080841.2 
TULYM-TT 2 PTPRB ATAGTGATCAAAGTCTCCAG 2921bp 4/4 NM_001109754.2
NM_001206971.1

NM_001206972.1 

NM_002837.4 

TULYM-TT 3 PTPRC GAAACTTGCTGAACACCCGC 2002bp 2/3 NM_002838.4

NM_080921.3

NM_001267798.1

TULYM-TT 4 PTPRD AAGCACCATCCAGTGCCCCG 1244bp 6/6 NM_002839.3 
NM_130391.3

NM_130392.3

NM_130393.3

NM_001171025.1
NM_001040712.2 
TULYM-TT 5 PTPRE CAGGAAGCAGAGGAAAGCTG 213bp 2/2 NM_006504.4
NM_130435.3  
TULYM-TT 6 PTPRF GACCAGACTGGGCTGTCAGG 121bp 2/2 NM_130440.2
NM_002840.3 
TULYM-TT 7 PTPRG GCAGCGCAGTGCAGATCCGC 131bp 1/1 NM_002841.3 
TULYM-TT 8 PTPRH GAGTACACTGGAGACGGTGG 463bp 2/2 NM_002842.4 
NM_001161440.2
TULYM-TT 9 PTPRJ GGAATAGGCAATGAGACTTG 565bp 2/2 NM_002843.3
NM_001098503.1 
TULYM-TT 10 PTPRK AAGAAGCTGAGGAGGAGCAA 903bp 6/6 NM_001291981.1
NM_001291982.1

NM_001291983.1 

NM_001291984.1 

NM_002844.3 

NM_001135648.2 

TULYM-TT 11 PTPRM TAGACATTGAGTAACCCCGG 350bp 2/2 NM_001105244.1 
NM_002845.3 
TULYM-TT 12 PTPRN GGAGCAGCTCAGCCTGCAGA 544bp 3/3 NM_002846.3

NM_001199764.1 

NM_001199763.1

TULYM-TT 13 PTPRN2 GCTCAGGAGGCCCTCAGCGA 1471bp 3/3 NM_002847.3

NM_130842.2 

NM_130843.2  

TULYM-TT 14 PTPRO GGAGTTGAAATTGATTGGAC 2745bp 6/6 NM_002848.3
NM_030667.2

NM_030668.2

NM_030669.2

NM_030670.2 

NM_030671.2 

TULYM-TT 15 PTPRQ AATGAAGACAAAATTTGCAA 5563bp 1/1 NM_001145026.1 
TULYM-TT 16 PTPRR TTGTGTCTATACCAACACCA 731bp 4/4 NM_001207015.1
NM_001207016.1

NM_002849.3

NM_130846.2 

TULYM-TT 18 PTPRT GGTACAGACCCACAAAGAGG 1672bp 2/2 NM_133170.3
NM_007050.5 
TULYM-TT 19 PTPRU AAGTGGCCAGGAAGCGCCGG 745bp 4/4 NM_001195001.1

NM_005704.4  

NM_133177.3

NM_133178.3 

TULYM-TT 20 PTPRV GATGCCCCCGGGAAGCAGGA 581bp 1/1 AJ629456
TULYM-TT 21 PTPRZ1 GTACCTTCCACAGTGTGAGG 1601bp 3/3 NM_001206838.1 

NM_001206839.1 

NM_002851.2 

TULYM-TT 22 PTPN1 GTCACTTTTGGGAGATGGTG 278bp 2/2 NM_002827.3 
NM_001278618.1 
TULYM-TT 23 PTPN2 GAACCCTGACCATGGGCCTG 615bp 4/4 NM_001207013.1

NM_002828.3  

NM_080422.2 

NM_080423.2 

TULYM-TT 25 PTPN4 GCAGCAGTATTAGAAGGACA 440bp 1/1 NM_002830.3
TULYM-TT 26 PTPN5 GCACCTGGTGCGGGAGGTGG 1416bp 6/6 NM_006906.1

NM_001278239.1 

NM_001278238.1

NM_001278236.1 

NM_001039970.1

NM_032781.3 
TULYM-TT 27 PTPN6 GATGTGGGTGACCCTGAGCG 501bp 3/3 NM_002831.5
NM_080548.4
NM_080549.3 
TULYM-TT 28 PTPN7 GCTGCGCCTAGTGGCAGAGG 1116bp 3/3 NM_002832.3
NM_001199797.1
NM_080588.2 
TULYM-TT 29 PTPN9 GCTAGAAATTCACAACACAG 1338bp 1/1 NM_002833.3 
TULYM-TT 30 PTPN11 TTTGTTCTTTCTGTGCGCAC 439bp 2/2 NM_002834.3
NM_080601.1 
TULYM-TT 31 PTPN12 AGAATAGAATCAAATGATGA 629bp 3/3 NM_002835.3 
NM_001131009.1
NM_001131008.1 
TULYM-TT 32 PTPN13 GTGAGAATGCAGCAGCTGAA 7024bp 4/4 NM_080683.2

NM_006264.2 

NM_080684.2

NM_080685.2

TULYM-TT 33 PTPN14 GAGGTTGGAGGATACAGGGG 1239bp 1/1 NM_005401.4 
TULYM-TT 34 PTPN18 ACTGTAGCTGGTACACAGAA 622bp 2/2 NM_014369.3
NM_001142370.1
TULYM-TT 35 PTPN20A ATGATAACCAGAGAGATAGA 757bp 4/10 NM_001042357.3 
NM_001042360.2

NM_001042363.3 

NM_001042358.3  

NM_001042361.3 

NM_001042365.2

NM_001042362.2  

NM_001042359.2

NM_015605.7

NM_001042364.3 
TULYM-TT 36 PTPN21 CAGTGAACCCAATCAGGAGG 950bp 1/1 NM_007039.3 
TULYM-TT 37 PTPN22 GTAGCTGGAATCCTCATCAG 252bp 3/3 NM_015967.5

NM_001193431.1 

NM_012411.4

TULYM-TT 38 PTPN23 TGAGGACATCAAGTACGAGC 321bp 1/1 NM_015466.2 
TULYM-TT 39 DUSP1 CGTCCAGCAACACCACGGCG 243bp 1/1 NM_004417.3 
TULYM-TT 40 DUSP2 CAGCAGGCAGGCGAGAACGG 213bp 1/1 NM_004418.3
short introns
TULYM-TT 41 DUSP4 GCACTGCCGAGGTAGAGGAA 623bp 2/2 NM_001394.6
NM_057158.3 
TULYM-TT 42 DUSP5 CAGCGAAGGCCAGATAGGGC 106bp 1/1 NM_004419.3 
TULYM-TT 43 DUSP6 GCTGCTGCTCAAGAAGCTCA 348bp 2/2 NM_001946.3 
NM_022652.3
TULYM-TT 44 DUSP7 TGGGCAGGTTGCCCTTGCGC 331bp 1/1 NM_001947.3 
TULYM-TT 45 DUSP8 GCTTGCCCTCGCAGAGGCCG 240bp 1/1 NM_004420.2 
TULYM-TT 46 DUSP9 CAGTCCAGGAGCAGGAGCCG 80bp 1/1 NM_001395.3
TULYM-TT 47 DUSP10 GCAGGTGGTAGAGGTTCCGA 336bp 1/1 NM_007207.5 
TULYM-TT 48 DUSP16 GAGAAAGCAAAAGCCTCCAA 691bp 1/1 NM_030640.2 
TULYM-TT 50 DUSP3 CAGGCTTTGGCTCAAAAGAA 331bp 1/1 NM_004090.3 
TULYM-TT 51 DUSP11 ACTATCTCCCAGTTGGACAG 251bp 1/1 NM_003584.2 
TULYM-TT 52 DUSP12 GAGGCTCCGGGCCCGAGTGA 7bp 1/1 NM_007240.1 
TULYM-TT 53 DUSP13A AAGAAGACTTCCCTGCCCGC 103bp 2/4 NM_001007271.1
NM_001007272.1
NM_001007273.1
NM_016364.3  
TULYM-TT 54 DUSP13B GAGATGCCTGGACTGCCCCA 64bp 3/4 NM_001007271.1 
NM_001007272.1
NM_001007273.1
NM_016364.3  
TULYM-TT 55 DUSP14 GCCCCTCGGATGATTTCCGA 43bp 1/1 NM_007026 
TULYM-TT 56 DUSP15 CTGTGACGGGGCTAGGCTGG 314bp 2/2 NM_080611.3
NM_177991.1
TULYM-TT 57 DUSP18 CCTGCTTCATCTCCACGCTG 292bp 1/1 NM_152511.3 
TULYM-TT 58 DUSP19 TCTCAGGAAGCAATGCACCA 45bp 2/2 NM_080876.3
NM_001142314.1 
TULYM-TT 59 DUSP21 CTTGTTTCTCAGCAATGGTG 84bp 1/1 NM_022076.3 
TULYM-TT 60 DUSP22 ATACCTGTGCATCCCAGCAG 166bp 2/2 NM_001286555.1 
NM_020185.4 
TULYM-TT 61 DUSP23 GAAGCACCCAGGAGAAGTTG 37bp 1/1 NM_017823.3 
TULYM-TT 62 STYXL1 GTGCCAGGGAAGGTCTTCGT 509bp 1/1 NM_016086.2 
TULYM-TT 64 DUSP26 CATGAGCATCCACTTCCAGA 375bp 1/1 NM_024025.1 
TULYM-TT 65 DUSP27 CCTGTGTCCTGGACCTACAG 338bp 1/1 NM_001080426.1 
TULYM-TT 66 EPM2A GCTCGGGGAGGTGGAGCTGG 180bp 2/2 NM_005670.3 
NM_001018041.1 
TULYM-TT 67 RNGTT CGAAATGACATAGAAAAAGA 226bp ’3/3 NM_003800.4
NM_001286428.1
NM_001286426.1
TULYM-TT 68 STYX CTACCTGTACTACAGAAACA 145bp 2/2 NM_145251.3
NM_001130701.1
TULYM-TT 69 SSH1 GGACCGGGTCCGGTACATGG 306bp ’3/3 NM_018984.3
NM_001161331.1
NM_001161330.1 
TULYM-TT 70 SSH2 TTAGAGGACTTACAGAACCG 1057bp 2/4 NM_001282129.1
NM_001282130.1
NM_001282131.1
NM_033389.3   
TULYM-TT 71 SSH3 GAAGGAGTCGACTCCAGCGA 83bp 1/1 NM_017857.3 
TULYM-TT 73 PTP4A2 GGTGGAGCTCCATCATCAAA 218bp 3/3 NM_080391.3
NM_001195101.1
NM_001195100.1 
TULYM-TT 74 PTP4A3 GAAGTACGGGGCTACCACTG 114bp 2/2 NM_032611.2
NM_007079.3
TULYM-TT 75 CDC14A TGGACCGCTGAACTTGGCAA 156bp 3/3 NM_003672.3

NM_033312.2  

NM_033313.2

TULYM-TT 76 CDC14B TCTTTGACAATGGCATCAGT 893bp 3/3 NM_033331.2
NM_003671.3
NM_001077181.1
TULYM-TT 77 CDKN3 ATTTGTTTTCTGCACCAGAG 225bp 2/2 NM_005192.3
NM_001130851.1  
TULYM-TT 78 PTPDC1 TAGCATGCTCACCAGGGCGC 541bp 4/4 NM_152422.4
NM_177995.2

NM_001253830.1 

NM_001253829.1 

TULYM-TT 79 PTEN ACCGCCAAATTTAATTGCAG 232bp 1/1 NM_000314.4 
TULYM-TT 80 TPTE2 AAAGACTGCCTTCCAGAAGA 722bp 4/4 NM_199254.2
NM_130785.3

NM_001271850.1

NM_001141968.1

TULYM-TT 81 TPTE TTGAAAAGCTGATAAGAAGG 644bp 4/4 NM_199261.3

NM_199260.3 

NM_199259.3

NM_001290224.1 
TULYM-TT 82 TNS1 GTAGAACAACGACATTGTGA 315bp 1/1 NM_022648.4
TULYM-TT 83 TENC1 GGGCCGCGCGGGGAAGGCGG 477bp 3/3 NM_015319.2
NM_170754.2
NM_198316.1 
TULYM-TT 84 MTM1 AGTGTTTCTCCTGGAAGTCG 128bp 1/1 NM_000252.2
TULYM-TT 85 MTMR1 CCATTCAACATGTGATCCTG 171bp 1/1 NM_003828.2
TULYM-TT 86 MTMR2 AAAGTCAAGCCACAATCACT 827bp 4/4 NM_016156.5 

NM_001243571.1 

NM_201278.2

NM_201281.2
TULYM-TT 87 MTMR3 TCAGAAGCACAGACAAGTGA 1177bp 3/3 NM_021090.3 
NM_153050.2
NM_153051.2  
TULYM-TT 88 MTMR4 GCACCAGGCATGGTAGGCAA 408bp 1/1 NM_004687.4 
TULYM-TT 89 SBF1 GAGGAAACAGCAGTGCCAGG 778bp 1/1 NM_002972.2 
TULYM-TT 90 MTMR6 CCGGTTTGGAGAGCTCGGGA 899bp 1/1 NM_004685.3 
TULYM-TT 91 MTMR7 GCAAATCGTGCTGCAGGGAA 745bp 1/1 NM_004686.4 
TULYM-TT 92 MTMR8 GCGGCCTGGAGAGCTCAGGG 905bp 1/1 NM_017677.3 
TULYM-TT 93 MTMR9 GAAAGGGTACATCAGAGTGA 333bp 1/1 NM_015458.3 
TULYM-TT 94 MTMR10 GGAATTCCCTCAGGAGATGG 580bp 1/1 NM_017762.2 
TULYM-TT 95 MTMR11 AATTCCTCTCATGGAGACAG 576bp 2/2 NM_001145862.1
NM_181873.3  
TULYM-TT 96 MTMR12 AAAGCAGTGAGTGTCAACGA 673bp 3/3 NM_001294343.1
NM_001040446.2
NM_001294344.1 
TULYM-TT 97 SBF2 GGACTGCACAAAAGAGGCTG 637bp 1/1 NM_030962.3 
TULYM-TT 98 MTMR14 TCCAGGAACACGATGTGCCG 278bp 3/3 NM_001077525.2

NM_001077526.2 

NM_022485.4

TULYM-TT 99 FAN1 ACTGTGGATTTGGAACTCTG 563bp 4/4 NM_014967.4
NM_001146096.1
NM_001146095.1
NM_001146094.1
TULYM-TT 100 ACP1 ACCCAGACACACAAACAGCA 26bp 3/3 NM_007099.3 
NM_004300.3
NM_001040649.2  
TULYM-TT 101 CDC25A GAACTTGCACATGGAAGAAG 1200bp 2/2 NM_001789.2
NM_201567.1 
TULYM-TT 102 CDC25B CGCCCGTGCAGAATAAGCGG 1031bp 10/10 NM_021873.3 
NM_021872.3

NM_004358.4

NM_001287524.1 

NM_001287522.1 

NM_001287520.1 

NM_001287519.1 

NM_001287518.1

NM_001287517.1
NM_001287516.1 
TULYM-TT 103 CDC25C GACATCTGGACAGACGGTAA 135bp 4/4 NM_001790.4 

NM_022809.3

NM_001287583.1

NM_001287582.1
TULYM-TT 104 EYA1 GATGGGAAATCACGTGGACG 898bp 6/6 NM_000503.5 

NM_001288574.1 

NM_001288575.1

NM_172058.3 

NM_172059.3  

NM_172060.3 

TULYM-TT 105 EYA2 GAGGTGGGTAAGCTGTATAG 268bp 2/2 NM_005244.4 
NM_172110.3
TULYM-TT 106 EYA3 AACATGACTAGCAAGAACCG 841bp 4/4 NM_001990.3 
NM_001282562.1
NM_001282561.1
NM_001282560.1 
TULYM-TT 107 EYA4 ACATCGAATAACACAGCCGA 598bp 5/5 NM_004100.4

NM_001301013.1 

NM_001301012.1

NM_172103.3 

NM_172105.3










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